Nucleic Acids - Mock Test 20

Question 191

In addition to Taq polymerase, polymerase chain reaction requires all of the following except?

A) A template DNA B) Deoxyribonucleoside triphosphates C) Primers D) Primase

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Question 192

From a DNA-RNA hybrid, DNA can be obtained by addition of?

A) DNA B protein and ATP B) Helicase and ATP C) DNA topoisomerase I D) Alkali

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Question 193

Base sequence of DNA can be determined by?

A) Maxam-Gilbert method B) Sanger’s dideoxy method C) Both A and B D) None of these

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Question 194

The DNA polymerase commonly used in polymerase chain reaction is obtained from?

A) E. coli B) Yeast C) T.aquaticus D) Eukaryotes

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Question 195

Polymerase chain reaction can rapidly amplify DNA sequences of the size?

A) Upto 10 kilobases B) Upto 45 kilobases C) Upto 100 kilobases D) Upto 1,000 kilobases

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Question 196

A cosmid is a?

A) Large bacterial plasmid B) Viral plasmid C) Hybrid of plasmid and phage D) Yeast plasmid

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Question 197

DNA fragments upto 45 kilobases in size can be cloned in?

A) Bacterial plasmids B) Lambda phage C) Cosmids D) Yeast artificial chromosomes

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Question 198

Lambda phage can be used to clone DNA fragments of the size?

A) Upto 3 kilobases B) Upto 20 kilobases C) Upto 45 kilobases D) Upto 1,000 kilobases

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Question 199

The plasmid pBR322 has?

A) Ampicillin resistance gene B) Tetracycline resistance gene C) Both A and B D) None of these

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Question 200

Which of the following may be used as a cloning vector?

A) Prokaryotic plasmid B) Lambda phage C) Cosmid D) All of these

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